The Rat TPO (Thrombopoietin) CLIA Kit is specifically designed for the quantitative measurement of thrombopoietin levels in rat serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and accuracy, ensuring precise and consistent results for research purposes.Thrombopoietin is a key cytokine that regulates platelet production and plays a crucial role in hematopoiesis. Abnormal TPO levels have been associated with various hematological disorders such as thrombocytopenia and myeloproliferative neoplasms.
Therefore, studying TPO levels can provide valuable insights into these conditions and help in the development of potential therapeutic interventions.With its advanced technology and robust performance, the Rat TPO CLIA Kit is an essential tool for researchers studying platelet biology, hematopoiesis, and related diseases in rat models. Get reliable and precise data with this innovative kit from AssayGenie.
Product Name:
Rat TPO (Thrombopoietin) CLIA Kit
SKU:
RTES00547
Target:
Rat TPO (Thrombopoietin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TPO. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TPO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TPO, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TPO. You can calculate the concentration of Rat TPO in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-108
99-116
99-114
Average (%)
103
106
105
1:4
Range (%)
86-99
87-101
96-110
Average (%)
91
93
104
1:8
Range (%)
102-116
102-119
85-98
Average (%)
109
110
90
1:16
Range (%)
94-109
87-101
100-113
Average (%)
100
93
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-109
104
EDTA plasma (n=5)
97-110
105
Cell culture media (n=5)
97-114
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.13
73.34
204.76
25.88
75.88
211.42
Standard deviation
1.98
5.31
14.87
2.79
5.87
21.14
C V (%)
8.21
7.24
7.26
10.78
7.74
10.0
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TPO concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TPO in samples. No significant cross-reactivity or interference between Rat TPO and analogues was observed.
