Rat TRACP-5b (Tartrate-Resistant Acid Phosphatase 5b) CLIA Kit
The Rat TRACP-5b (Tartrate Resistant Acid Phosphatase 5b) CLIA Kit is a highly reliable assay designed for the quantitative detection of TRACP-5b levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.TRACP-5b is an important marker for bone metabolism, with elevated levels commonly seen in conditions such as osteoporosis and bone metastases.
Monitoring TRACP-5b levels can provide valuable insights into bone health and the efficacy of potential osteoporosis treatments.With its high performance and ease of use, the Rat TRACP-5b CLIA Kit is an essential tool for researchers studying bone metabolism and related disorders in rat models. Get reliable and informative results with this advanced assay kit from AssayGenie.
Product Name:
Rat TRACP-5b (Tartrate-Resistant Acid Phosphatase 5b) CLIA Kit
SKU:
RTES00536
Target:
Rat TRACP-5b (Tartrate-Resistant Acid Phosphatase 5b)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TRACP-5b. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TRACP-5b and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TRACP-5b, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TRACP-5b. You can calculate the concentration of Rat TRACP-5b in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-100
99-114
87-99
Average (%)
93
106
92
1:4
Range (%)
86-101
93-111
88-103
Average (%)
92
101
94
1:8
Range (%)
103-115
101-116
85-97
Average (%)
109
107
91
1:16
Range (%)
90-106
91-103
85-99
Average (%)
97
97
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
99
EDTA plasma (n=5)
100-113
106
Cell culture media (n=5)
85-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
386.46
1004.83
2812.95
362.59
1069.66
2642.36
Standard deviation
34.32
109.83
193.81
34.34
89.64
251.82
C V (%)
8.88
10.93
6.89
9.47
8.38
9.53
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TRACP-5b concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TRACP-5b in samples. No significant cross-reactivity or interference between Rat TRACP-5b and analogues was observed.
