Rat TRAIL/TNFSF10 (Tumor Necrosis Factor Related Apoptosis Inducing Ligand) CLIA Kit
The Rat TRAIL (TNFSF10) Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand CLIA Kit is specifically designed for the quantitative measurement of TRAIL levels in rat serum, plasma, and tissue homogenates. This CLIA kit offers superior sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.TRAIL, also known as TNFSF10, is a key protein involved in apoptosis induction and immune surveillance against tumor cells.
It plays a crucial role in cancer therapy and has been studied for its potential in targeted cancer treatment. This CLIA kit provides researchers with a valuable tool for studying the role of TRAIL in cancer progression and developing novel therapeutic strategies.
Product Name:
Rat TRAIL/TNFSF10 (Tumor Necrosis Factor Related Apoptosis Inducing Ligand) CLIA Kit
SKU:
RTES00580
Target:
Rat TRAIL/TNFSF10 (Tumor Necrosis Factor Related Apoptosis Inducing Ligand)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TRAIL/TNFSF10. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TRAIL/TNFSF10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TRAIL/TNFSF10, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TRAIL/TNFSF10. You can calculate the concentration of Rat TRAIL/TNFSF10 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
99-115
102-118
Average (%)
100
105
109
1:4
Range (%)
85-100
85-97
100-116
Average (%)
91
91
109
1:8
Range (%)
84-95
97-111
95-109
Average (%)
90
105
103
1:16
Range (%)
98-112
87-101
99-115
Average (%)
104
93
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-98
91
EDTA plasma (n=5)
91-104
98
Cell culture media (n=5)
95-108
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.07
75.06
237.53
21.72
67.98
244.15
Standard deviation
2.44
5.56
14.73
1.77
7.51
18.78
C V (%)
10.58
7.41
6.2
8.15
11.05
7.69
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TRAIL/TNFSF10 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TRAIL/TNFSF10 in samples. No significant cross-reactivity or interference between Rat TRAIL/TNFSF10 and analogues was observed.
