Rat Transforming Growth Factor Beta 3 (TGFb3) ELISA Kit
The Rat Transforming Growth Factor Beta 3 (TGFB3) ELISA Kit is specifically designed for the accurate quantification of TGFB3 levels in rat serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring precise and consistent results that are essential for various research applications.TGFB3 is a key protein involved in cell growth, differentiation, and development. It plays a crucial role in processes such as tissue regeneration, immune response modulation, and wound healing. Dysregulation of TGFB3 has been linked to various diseases, including cancer, fibrosis, and autoimmune disorders, highlighting its importance as a biomarker for studying disease pathogenesis and potential therapeutic interventions.
With the Rat TGFB3 ELISA Kit, researchers can accurately measure TGFB3 levels in biological samples, enabling in-depth analysis of its biological functions and molecular mechanisms. This kit provides a reliable tool for advancing research in fields such as molecular biology, immunology, and pharmacology.
Product Name:
Rat Transforming Growth Factor Beta 3 (TGFb3) ELISA Kit
Product Code:
RTDL01020
Size:
96 Assays
Target:
TGFb3
Synonyms:
TGF-B3, LAP, Latency-associated peptide
Detection Method:
Sandwich
Range:
15.625-1000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-99
91
EDTA plasma (n=5)
80-99
89
Heparin plasma (n=5)
82-99
90
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
