The Rat Transgelin (TAGLN) ELISA Kit is specifically designed for the quantitative detection of transgelin levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing accurate and reliable results for a variety of research applications.Transgelin, also known as smooth muscle protein 22-alpha (SM22-alpha), is a key protein involved in regulating smooth muscle contraction and cell migration. It plays a critical role in various physiological processes, including wound healing, vascular remodeling, and fibrosis.
Dysregulation of transgelin expression has been implicated in diseases such as cancer, cardiovascular disorders, and respiratory diseases, making it a valuable biomarker for studying these conditions and developing potential therapies.Overall, the Rat Transgelin ELISA Kit is a valuable tool for researchers looking to study the role of transgelin in health and disease, providing accurate and reproducible results to advance our understanding of this important protein.
Product Name:
Rat Transgelin (TAGLN) ELISA Kit
Product Code:
RTDL01003
Size:
96 Assays
Target:
TAGLN
Synonyms:
SMCC, SM22, TAGLN1, WS3-10, Transgelin Variant 2, 22 kDa actin-binding protein, Protein WS3-10, Smooth muscle protein 22-alpha
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
