Rat Transient Receptor Potential Cation Channel Subfamily M, Member 7 (TRPM7) ELISA Kit
The Rat TRPM7 (Transient Receptor Potential Cation Channel Subfamily M Member 7) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of TRPM7 levels in rat serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an excellent tool for a variety of research applications.TRPM7 is a crucial ion channel protein that plays a vital role in regulating cellular magnesium and calcium levels, as well as influencing cellular processes such as cell proliferation, migration, and survival.
Dysregulation of TRPM7 has been linked to various diseases, including cancer, cardiovascular disorders, and neurological conditions, making it an important biomarker for studying these diseases and exploring potential therapeutic interventions.Overall, the Rat TRPM7 ELISA Kit offers researchers a powerful tool for accurately measuring TRPM7 levels in biological samples, facilitating in-depth studies on the role of TRPM7 in health and disease.
Product Name:
Rat Transient Receptor Potential Cation Channel Subfamily M, Member 7 (TRPM7) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
