The Rat Troponin I Type 3 Cardiac (TNNI3) ELISA Kit is a reliable and accurate tool for measuring TNNI3 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.TNNI3 is a critical protein found in cardiac muscle, playing a vital role in muscle contraction and relaxation. Elevated levels of TNNI3 in the blood are indicative of cardiac injury or stress, making it a valuable biomarker for studying cardiovascular diseases, myocardial infarction, and other cardiac conditions.
With the Rat Troponin I Type 3 Cardiac (TNNI3) ELISA Kit, researchers can accurately measure TNNI3 levels in biological samples, providing valuable insights into cardiac health and potential therapeutic interventions.
Product Name:
Rat Troponin I Type 3, Cardiac (TNNI3) ELISA Kit
Product Code:
RTDL01049
Size:
96 Assays
Target:
TNNI3
Synonyms:
cTnI, TNNC1, CMH7, C-TnI, Troponin I Type 3, Cardiac troponin I
Detection Method:
Sandwich
Range:
31.25-2,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
