The Rat Troponin T Type 2 Cardiac (TNNT2) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of TNNT2 levels in rat serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for various research applications.Troponin T Type 2 Cardiac is a key protein found in cardiac muscle, playing a crucial role in regulating muscle contraction and relaxation. Elevated levels of TNNT2 are associated with cardiac injury and can serve as a biomarker for conditions such as myocardial infarction and heart failure.
The Rat TNNT2 ELISA Kit is a valuable tool for studying these cardiac conditions and developing potential therapeutic interventions.Overall, the Rat Troponin T Type 2 Cardiac (TNNT2) ELISA Kit offers researchers a reliable and accurate method for measuring TNNT2 levels in rat samples, enabling further exploration into the role of this protein in cardiac health and disease.
Product Name:
Rat Troponin T Type 2, Cardiac (TNNT2) ELISA Kit
Product Code:
RTDL01051
Size:
96 Assays
Target:
TNNT2
Synonyms:
CMH2, cTnT, TnTc, Cardiomyopathy,Hypertrophic 2, Cardiac muscle troponin T
Detection Method:
Sandwich
Range:
15.625-1,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
