The Rat TRXR1 (Thioredoxin Reductase 1) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of TRXR1 levels in rat samples, including serum, plasma, and tissue lysates. This kit provides reliable and reproducible results, making it ideal for research applications in the fields of oxidative stress, redox signaling, and antioxidant defense mechanisms.TRXR1 is an important enzyme involved in redox regulation and plays a crucial role in maintaining cellular homeostasis and protecting cells from oxidative damage. Dysregulation of TRXR1 has been implicated in various diseases, including cancer, cardiovascular diseases, and neurological disorders.
Therefore, the Rat TRXR1 CLIA Kit is a valuable tool for studying the role of TRXR1 in these conditions and developing potential therapeutic strategies.Overall, the Rat TRXR1 CLIA Kit is a powerful research tool that offers high sensitivity and accuracy for the quantitative analysis of TRXR1 levels in rat samples, providing valuable insights into the molecular mechanisms underlying oxidative stress-related diseases.
Product Name:
Rat TrxR1 (Thioredoxin Reductase 1) CLIA Kit
SKU:
RTES00542
Target:
Rat TrxR1 (Thioredoxin Reductase 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TrxR1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TrxR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TrxR1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TrxR1. You can calculate the concentration of Rat TrxR1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-98
90-104
92-102
Average (%)
93
96
97
1:4
Range (%)
95-107
92-104
96-109
Average (%)
101
97
101
1:8
Range (%)
85-100
102-117
97-113
Average (%)
91
109
103
1:16
Range (%)
98-109
99-113
83-96
Average (%)
104
106
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-108
98
EDTA plasma (n=5)
88-104
95
Cell culture media (n=5)
98-115
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
94.87
312.86
818.22
103.91
340.67
786.92
Standard deviation
9.16
24.0
87.71
11.8
36.96
73.58
C V (%)
9.66
7.67
10.72
11.36
10.85
9.35
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TrxR1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TrxR1 in samples. No significant cross-reactivity or interference between Rat TrxR1 and analogues was observed.
