Rat TSGF (Tumor Specific Growth Factor/Tumor Supplied Group of Factor) CLIA Kit
The Rat TSGF (Tumor Specific Growth Factor) CLIA Kit is a specialized assay designed for the precise measurement of TSGF levels in rat tumor samples. This kit offers high accuracy and sensitivity, providing consistent and dependable results for a variety of research purposes.TSGF is a key factor in tumor development, contributing to cell growth and proliferation within tumor tissues. By accurately measuring TSGF levels, researchers can gain valuable insights into the progression of tumors and potential treatment options.
This CLIA kit is essential for studying the role of TSGF in cancer biology and identifying potential therapeutic targets for inhibiting tumor growth. With its reliable performance and ease of use, the Rat TSGF CLIA Kit is a valuable tool for advancing cancer research and drug discovery efforts.
Product Name:
Rat TSGF (Tumor Specific Growth Factor/Tumor Supplied Group of Factor) CLIA Kit
SKU:
RTES00582
Target:
Rat TSGF (Tumor Specific Growth Factor/Tumor Supplied Group of Factor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TSGF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TSGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TSGF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TSGF. You can calculate the concentration of Rat TSGF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-99
88-101
102-119
Average (%)
91
95
108
1:4
Range (%)
94-107
88-101
96-108
Average (%)
102
95
103
1:8
Range (%)
91-104
103-119
95-107
Average (%)
96
110
100
1:16
Range (%)
99-114
96-109
94-108
Average (%)
106
103
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
99
EDTA plasma (n=5)
99-114
106
Cell culture media (n=5)
87-102
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
97.54
269.33
832.26
89.6
295.81
859.76
Standard deviation
9.47
31.13
53.93
9.26
26.15
98.53
C V (%)
9.71
11.56
6.48
10.33
8.84
11.46
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TSGF concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TSGF in samples. No significant cross-reactivity or interference between Rat TSGF and analogues was observed.
