The Rat TSP-1 (Thrombospondin-1) CLIA Kit is a high-quality assay designed for the precise measurement of thrombospondin-1 levels in rat samples such as serum, plasma, and tissue homogenates. With its exceptional sensitivity and specificity, this kit delivers reliable and consistent results, making it a valuable tool for various research applications.Thrombospondin-1 is a vital protein known for its role in regulating cell-matrix interactions, inflammation, and angiogenesis.
Its aberrant expression has been linked to various diseases, including cancer, cardiovascular disorders, and fibrosis, highlighting its significance as a potential biomarker for disease diagnosis and therapeutic development.With the Rat TSP-1 CLIA Kit, researchers can accurately quantify thrombospondin-1 levels in rat samples, advancing our understanding of its biological functions and potential therapeutic targets in disease settings.
Product Name:
Rat TSP-1 (Thrombospondin 1) CLIA Kit
SKU:
RTES00548
Target:
Rat TSP-1 (Thrombospondin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TSP-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TSP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TSP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TSP-1. You can calculate the concentration of Rat TSP-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-114
103-120
92-106
Average (%)
105
110
97
1:4
Range (%)
93-106
95-108
94-110
Average (%)
101
102
101
1:8
Range (%)
100-118
97-110
96-108
Average (%)
108
104
102
1:16
Range (%)
101-119
89-101
92-107
Average (%)
110
95
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-104
95
EDTA plasma (n=5)
90-103
96
Cell culture media (n=5)
90-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
106.5
303.64
890.0
97.62
328.13
886.54
Standard deviation
13.6
28.09
67.82
7.97
36.98
79.17
C V (%)
12.77
9.25
7.62
8.16
11.27
8.93
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TSP-1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TSP-1 in samples. No significant cross-reactivity or interference between Rat TSP-1 and analogues was observed.
