Rat Tumor Necrosis Factor Ligand Superfamily, Member 12 (TNFSF12) ELISA Kit
The Rat Tumor Necrosis Factor Ligand Superfamily Member 12 (TNFSF12) ELISA Kit is specifically designed for the precise measurement of TNFSF12 levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.TNFSF12, also known as Tumor Necrosis Factor Ligand Superfamily Member 12, is a key signaling protein involved in immune response regulation and cell death. It plays a critical role in various disease states, including inflammation, autoimmune disorders, and cancer progression, making it a valuable biomarker for investigating these conditions and potential therapeutic interventions.
Researchers can rely on the Rat TNFSF12 ELISA Kit to effectively measure TNFSF12 levels in rat samples, providing valuable insights into pathophysiological mechanisms and disease progression. This kit is a valuable tool for advancing scientific knowledge and developing targeted therapies for TNFSF12-related disorders.
Product Name:
Rat Tumor Necrosis Factor Ligand Superfamily, Member 12 (TNFSF12) ELISA Kit
Product Code:
RTDL01046
Size:
96 Assays
Target:
TNFSF12
Synonyms:
APO3L, DR3LG, TWEAK, APO3 ligand, TNF-related weak inducer of apoptosis
Detection Method:
Sandwich
Range:
78.125-5000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-98
94
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
