The Rat TYR (Tyrosinase) CLIA Kit is specifically designed to detect levels of tyrosinase in rat samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing accurate and reliable results for a variety of research applications.Tyrosinase is an enzyme that plays a vital role in melanin production, making it an important target for studying pigmentation disorders and skin conditions.
By accurately measuring tyrosinase levels, researchers can gain valuable insights into these conditions and potentially develop new treatments.Overall, the Rat TYR (Tyrosinase) CLIA Kit is a valuable tool for researchers looking to investigate the role of tyrosinase in various biological processes and diseases, providing essential data for advancing scientific knowledge and potential therapeutic development.
Product Name:
Rat TYR (Tyrosinase) CLIA Kit
SKU:
RTES00583
Target:
Rat TYR (Tyrosinase)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TYR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TYR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TYR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TYR. You can calculate the concentration of Rat TYR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-106
98-114
91-107
Average (%)
97
105
98
1:4
Range (%)
86-100
98-112
92-104
Average (%)
92
104
99
1:8
Range (%)
94-109
99-115
91-105
Average (%)
100
107
98
1:16
Range (%)
97-111
96-110
100-117
Average (%)
104
101
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
92
EDTA plasma (n=5)
97-113
103
Cell culture media (n=5)
84-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.98
63.15
200.54
21.73
61.18
210.0
Standard deviation
2.91
6.25
22.6
1.81
4.58
12.73
C V (%)
12.14
9.9
11.27
8.33
7.49
6.06
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TYR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TYR in samples. No significant cross-reactivity or interference between Rat TYR and analogues was observed.
