The Rat Tyrosine Hydroxylase (TH) ELISA Kit is specifically designed for the accurate quantification of tyrosine hydroxylase levels in rat samples, including serum, plasma, and tissue lysates. This kit offers high sensitivity and specificity, ensuring precise and reliable results for researchers working in the field of neuroscience and neurodegenerative disorders.Tyrosine hydroxylase is a key enzyme in the synthesis of dopamine, norepinephrine, and epinephrine, playing a crucial role in the regulation of neurotransmitter levels and motor functions.
Imbalances in tyrosine hydroxylase levels have been associated with various neurological disorders, including Parkinson's disease and schizophrenia, making it a valuable target for therapeutic interventions and biomarker studies.With its ease of use and high-performance capabilities, the Rat Tyrosine Hydroxylase (TH) ELISA Kit is an essential tool for researchers looking to explore the role of tyrosine hydroxylase in neurobiology and neurodegenerative diseases.
Product Name:
Rat Tyrosine Hydroxylase (TH) ELISA Kit
Product Code:
RTDL01022
Size:
96 Assays
Target:
TH
Synonyms:
TYH, DYT5b, Tyrosine 3-Monooxygenase, Dystonia 14
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
