Rat UCHL1 (Ubiquitin Carboxyl Terminal Hydrolase L1) ELISA Kit
The Rat UCHL1 (Ubiquitin Carboxyl-Terminal Hydrolase L1) ELISA Kit is specifically designed for the accurate quantification of UCHL1 levels in rat samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it an excellent tool for a variety of research applications.UCHL1 is a critical enzyme responsible for regulating protein degradation and maintaining cellular homeostasis. Dysregulation of UCHL1 has been implicated in various diseases, including neurodegenerative disorders and cancer.
Therefore, measuring UCHL1 levels can provide valuable insights into the pathogenesis of these conditions and potentially lead to the development of targeted therapies.Overall, the Rat UCHL1 ELISA Kit is an essential tool for researchers investigating the role of UCHL1 in health and disease, offering accurate and precise measurements to advance our understanding of this important enzyme.
Product Name:
Rat UCHL1 (Ubiquitin Carboxyl Terminal Hydrolase L1) ELISA Kit
SKU:
RTES01096
Target:
Rat UCHL1 (Ubiquitin Carboxyl Terminal Hydrolase L1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat UCHL1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat UCHL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat UCHL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat UCHL1. You can calculate the concentration of Rat UCHL1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-109
97-115
95-107
Average (%)
104
105
102
1:4
Range (%)
88-102
79-90
86-99
Average (%)
95
85
93
1:8
Range (%)
87-99
83-95
87-103
Average (%)
94
87
94
1:16
Range (%)
91-105
85-98
81-93
Average (%)
97
92
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-109
100
EDTA plasma (n=5)
93-108
100
Cell culture media (n=5)
84-100
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
200.02
430.69
1489.86
208.52
445.85
1600.11
Standard deviation
11.66
25.63
51.55
14.39
21.31
64.64
C V (%)
5.83
5.95
3.46
6.9
4.78
4.04
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat UCHL1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat UCHL1 in samples. No significant cross-reactivity or interference between Rat UCHL1 and analogues was observed.
