The Rat Urocortin-3 (UCN3) ELISA Kit from Assay Genie is a powerful tool for accurately measuring UCN3 levels in rat serum, plasma, and tissue culture supernatants. With its exceptional sensitivity and specificity, this kit provides dependable and consistent results, making it an excellent choice for a variety of research applications.UCN3 is a vital peptide hormone that is involved in regulating stress responses, energy balance, and cardiovascular function. Its dysregulation has been linked to various disorders, including anxiety, depression, and metabolic syndromes.
By using the Rat UCN3 ELISA Kit, researchers can gain valuable insights into the role of UCN3 in these conditions and potentially identify new therapeutic targets.Overall, the Rat UCN3 ELISA Kit offers researchers a reliable and accurate method for quantifying UCN3 levels in rat samples, advancing our understanding of the physiological and pathological roles of this important hormone.
Product Name:
Rat Urocortin 3 (UCN3) ELISA Kit
Product Code:
RTDL01079
Size:
96 Assays
Target:
UCN3
Synonyms:
SCP, SPC, UCNIII, Stresscopin
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
