Rat V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN) ELISA Kit
The Rat v-Jun Sarcoma Virus 17 Oncogene Homolog (JUN) ELISA Kit is a powerful tool for the quantitative detection of v-Jun Sarcoma Virus 17 Oncogene levels in rat samples, including serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a wide range of research applications.The v-Jun Sarcoma Virus 17 Oncogene, also known as JUN, is a key player in cell transformation and cancer development. Its aberrant activation has been linked to various types of cancers, making it a valuable biomarker for studying tumorigenesis and potential therapeutic interventions.
By using the Rat v-Jun Sarcoma Virus 17 Oncogene Homolog (JUN) ELISA Kit, researchers can gain valuable insights into the role of JUN in cancer biology and potentially identify novel targets for cancer treatment. This kit is easy to use and provides reliable results, making it an essential tool for cancer research studies.
Product Name:
Rat V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN) ELISA Kit
Product Code:
RTDL00599
Size:
96 Assays
Target:
JUN
Synonyms:
C-Jun, AP1, CJun, p39, Jun Oncogene, Proto-oncogene c-Jun, Activator protein 1, Transcription factor AP-1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
