Rat Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA Kit
The Rat Vascular Cell Adhesion Molecule-1 (VCAM1) ELISA Kit offered by Assay Genie is a reliable and accurate tool for measuring VCAM1 levels in rat samples. This kit is specifically designed for use with rat serum, plasma, and cell culture supernatants, providing researchers with the ability to study this important adhesion molecule in a variety of biological samples.VCAM1 is a key protein involved in cell adhesion and inflammation, playing a crucial role in processes such as leukocyte recruitment and vascular inflammation. Elevated levels of VCAM1 have been associated with various inflammatory conditions, making it a valuable biomarker for studying diseases like atherosclerosis, diabetes, and inflammatory disorders.
With its high sensitivity and specificity, the Rat VCAM1 ELISA Kit ensures accurate and reproducible results, making it an indispensable tool for researchers looking to explore the role of VCAM1 in various physiological and pathological processes. Invest in this ELISA kit today to advance your research in cardiovascular diseases, inflammation, and beyond.
Product Name:
Rat Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA Kit
Product Code:
RTDL01093
Size:
96 Assays
Target:
VCAM1
Synonyms:
CD106, INCAM-100, L1CAM
Detection Method:
Sandwich
Range:
12.5-800pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
