Rat VEGF-B (Vascular Endothelial Cell Growth Factor B) CLIA Kit
The Rat VEGF-B (Vascular Endothelial Cell Growth Factor B) CLIA Kit is a powerful tool for researchers looking to study the role of VEGF-B in vascular endothelial cell growth. This kit is specifically designed for the accurate and reliable detection of VEGF-B levels in rat serum, plasma, and cell culture supernatants.VEGF-B is a vital growth factor that plays a key role in angiogenesis, the process through which new blood vessels are formed. By promoting endothelial cell growth, VEGF-B helps regulate blood vessel development and function.
Dysregulation of VEGF-B has been implicated in various disease states, including cancer, cardiovascular diseases, and diabetes.With its high sensitivity and specificity, the Rat VEGF-B CLIA Kit provides trustworthy results that can aid in understanding the mechanisms underlying these diseases. This kit is an essential tool for researchers seeking to uncover the potential therapeutic implications of targeting VEGF-B in various pathological conditions.
Product Name:
Rat VEGF-B (Vascular Endothelial Cell Growth Factor B) CLIA Kit
SKU:
RTES00587
Target:
Rat VEGF-B (Vascular Endothelial Cell Growth Factor B)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat VEGF-B. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat VEGF-B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat VEGF-B, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat VEGF-B. You can calculate the concentration of Rat VEGF-B in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
93-106
97-112
Average (%)
103
100
104
1:4
Range (%)
88-99
86-101
89-99
Average (%)
94
92
94
1:8
Range (%)
102-119
93-104
99-115
Average (%)
109
99
106
1:16
Range (%)
86-101
97-115
96-110
Average (%)
92
105
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-112
104
EDTA plasma (n=5)
99-114
105
Cell culture media (n=5)
87-99
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.6
63.9
228.87
22.3
69.31
235.07
Standard deviation
2.34
7.04
23.25
2.78
5.61
24.14
C V (%)
9.51
11.02
10.16
12.47
8.09
10.27
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat VEGF-B concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat VEGF-B in samples. No significant cross-reactivity or interference between Rat VEGF-B and analogues was observed.
