Rat VEGF-D (Vascular Endothelial Cell Growth Factor D) CLIA Kit
The Rat VEGF-D (Vascular Endothelial Cell Growth Factor-D) CLIA Kit is a powerful tool for researchers looking to quantify levels of VEGF-D in rat samples including serum, plasma, and tissue lysates. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and reproducible results for various experimental settings.VEGF-D is a key player in the regulation of lymphangiogenesis and angiogenesis, crucial processes in tumor growth, metastasis, and inflammation. By accurately measuring VEGF-D levels, researchers can gain valuable insights into the mechanisms driving these pathological conditions and potentially identify novel therapeutic targets.
With its user-friendly protocol and high-performance capabilities, the Rat VEGF-D CLIA Kit is an invaluable resource for studying vascular biology, cancer research, and beyond. Invest in this kit to advance your research and uncover new possibilities in the field of angiogenesis and vascular biology.
Product Name:
Rat VEGF-D (Vascular Endothelial Cell Growth Factor D) CLIA Kit
SKU:
RTES00589
Target:
Rat VEGF-D (Vascular Endothelial Cell Growth Factor D)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat VEGF-D. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat VEGF-D and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat VEGF-D, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat VEGF-D. You can calculate the concentration of Rat VEGF-D in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-102
93-107
87-98
Average (%)
93
98
93
1:4
Range (%)
101-113
102-116
93-105
Average (%)
107
109
99
1:8
Range (%)
96-110
97-112
91-102
Average (%)
102
103
97
1:16
Range (%)
94-107
91-105
95-110
Average (%)
102
99
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-112
106
EDTA plasma (n=5)
99-115
107
Cell culture media (n=5)
95-111
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.02
59.99
232.71
29.43
55.28
229.79
Standard deviation
2.3
6.56
14.66
3.14
4.99
16.87
C V (%)
8.51
10.94
6.3
10.67
9.03
7.34
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat VEGF-D concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat VEGF-D in samples. No significant cross-reactivity or interference between Rat VEGF-D and analogues was observed.
