Rat VEGFR-2/KDR (Vascuoar Endothelial Growth Factor Receptor 2) CLIA Kit
The Rat VEGFR-2 (KDR) CLIA Kit is a powerful tool for the quantitative measurement of Vascular Endothelial Growth Factor Receptor 2 (VEGFR-2) in rat samples. This kit offers high sensitivity and accuracy, ensuring reliable and reproducible results for research purposes.VEGFR-2, also known as KDR, is a key receptor involved in angiogenesis and vascular endothelial cell growth. Its expression is essential for the formation of new blood vessels, making it a vital target in various disease conditions, including cancer, cardiovascular diseases, and inflammatory disorders.
By utilizing the Rat VEGFR-2 (KDR) CLIA Kit, researchers can effectively analyze VEGFR-2 levels in biological samples such as serum, plasma, and tissue extracts. This comprehensive kit provides the necessary tools to study the role of VEGFR-2 in angiogenesis and its potential as a therapeutic target in various pathological conditions.
Product Name:
Rat VEGFR-2/KDR (Vascuoar Endothelial Growth Factor Receptor 2) CLIA Kit
SKU:
RTES00590
Target:
Rat VEGFR-2/KDR (Vascuoar Endothelial Growth Factor Receptor 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat VEGFR-2/KDR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat VEGFR-2/KDR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat VEGFR-2/KDR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat VEGFR-2/KDR. You can calculate the concentration of Rat VEGFR-2/KDR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-108
101-120
92-105
Average (%)
102
109
97
1:4
Range (%)
90-105
99-115
96-113
Average (%)
97
106
103
1:8
Range (%)
88-103
90-101
96-107
Average (%)
95
96
102
1:16
Range (%)
93-107
100-115
93-107
Average (%)
100
108
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-110
100
EDTA plasma (n=5)
92-106
99
Cell culture media (n=5)
88-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.83
239.76
945.56
91.7
259.26
904.28
Standard deviation
10.79
25.1
59.19
11.29
28.0
55.88
C V (%)
11.26
10.47
6.26
12.31
10.8
6.18
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat VEGFR-2/KDR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat VEGFR-2/KDR in samples. No significant cross-reactivity or interference between Rat VEGFR-2/KDR and analogues was observed.
