The Rat VF (Visfatin) CLIA Kit is a reliable and accurate tool for measuring visfatin levels in rat samples. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications.Visfatin is an adipocytokine that plays a key role in metabolic regulation, inflammation, and insulin sensitivity. It is involved in various physiological processes, including energy metabolism and immune response. Abnormal visfatin levels have been linked to obesity, diabetes, and other metabolic disorders, making it a valuable biomarker for studying these conditions and developing potential treatment strategies.
The Rat VF (Visfatin) CLIA Kit is a valuable resource for researchers looking to investigate the role of visfatin in health and disease in rat models. Its user-friendly format and high performance make it a valuable addition to any laboratory working in the field of metabolic and inflammatory research.
Product Name:
Rat VF (Visfatin) CLIA Kit
SKU:
RTES00593
Target:
Rat VF (Visfatin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat VF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat VF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat VF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat VF. You can calculate the concentration of Rat VF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
88-100
103-120
Average (%)
101
94
110
1:4
Range (%)
102-116
94-108
83-96
Average (%)
108
100
90
1:8
Range (%)
98-113
93-107
92-104
Average (%)
107
99
97
1:16
Range (%)
94-108
93-107
86-100
Average (%)
102
100
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-111
102
EDTA plasma (n=5)
95-108
102
Cell culture media (n=5)
97-115
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.74
197.78
985.36
97.22
179.09
888.02
Standard deviation
11.93
22.07
103.36
10.32
18.25
58.96
C V (%)
12.46
11.16
10.49
10.62
10.19
6.64
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat VF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat VF in samples. No significant cross-reactivity or interference between Rat VF and analogues was observed.
