The Rat VLDL (Very Low-Density Lipoprotein) CLIA Kit is a cutting-edge assay designed for the precise measurement of VLDL levels in rat samples. With its exceptional sensitivity and specificity, this kit delivers dependable and consistent results, making it an invaluable tool for various research studies.VLDL is a key player in lipid metabolism, and abnormal levels of this lipoprotein are associated with various health conditions, including cardiovascular diseases and obesity.
By accurately measuring VLDL levels, researchers can gain valuable insights into the mechanisms underlying these diseases and explore potential therapeutic interventions.Overall, the Rat VLDL CLIA Kit is a reliable and efficient solution for studying VLDL metabolism and its implications on health and disease in rat models.
Product Name:
Rat VLDL (Very Low Density Lipoprotein) CLIA Kit
SKU:
RTES00608
Target:
Rat VLDL (Very Low Density Lipoprotein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat VLDL. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat VLDL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat VLDL, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat VLDL. You can calculate the concentration of Rat VLDL in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-112
103-118
93-106
Average (%)
102
109
98
1:4
Range (%)
88-100
96-108
91-102
Average (%)
94
103
96
1:8
Range (%)
91-105
101-118
100-119
Average (%)
99
108
108
1:16
Range (%)
94-106
104-118
102-116
Average (%)
101
110
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-104
95
EDTA plasma (n=5)
98-114
104
Cell culture media (n=5)
88-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
42.87
113.36
281.28
41.6
124.48
289.03
Standard deviation
4.11
9.37
29.84
4.38
12.1
17.57
C V (%)
9.59
8.27
10.61
10.53
9.72
6.08
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat VLDL concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat VLDL in samples. No significant cross-reactivity or interference between Rat VLDL and analogues was observed.
