The RBBP9 Antibody (CAB4451) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, shows high specificity and sensitivity for detecting RBBP9 in human samples, making it ideal for Western blot applications.RBBP9, also known as Retinoblastoma-binding protein 9, is involved in various cellular processes, including chromatin remodeling and transcriptional regulation. Its dysregulation has been implicated in cancer development and progression, making it a promising target for cancer research.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
RBBP9 Antibody
SKU:
CAB4451
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BOG, RBBP10, RBBP9
Positive Sample:
U-87MG, A375, LO2, Mouse kidney
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
21kDa
Observed MW:
18kDa
The protein encoded by this gene is a retinoblastoma binding protein that may play a role in the regulation of cell proliferation and differentiation. Two alternatively spliced transcript variants of this gene with identical predicted protein products have been reported, one of which is a nonsense-mediated decay candidate.
Purification Method
Affinity purification
Gene ID
10741
RRID
AB_2765685
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using RBBP9 Rabbit pAb (CAB4451) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Rat ovary using RBBP9 Rabbit pAb (CAB4451) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using RBBP9 Rabbit pAb (CAB4451) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using RBBP9 Rabbit pAb (CAB4451) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
