The REC8 Antibody (CAB15002) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity and specificity towards REC8 in human samples, making it suitable for Western blot applications. By binding to the REC8 protein, this antibody allows for precise detection and analysis in various cell types, providing valuable insights into meiotic processes and chromosomal segregation.REC8 is crucial for the proper alignment and segregation of chromosomes during meiosis, ensuring the accurate distribution of genetic material to daughter cells.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Rat samples.
Product Name:
REC8 Antibody
SKU:
CAB15002
Size:
20μL, 100μL
Reactivity:
Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
mrec, Rec8L1, REC8
Positive Sample:
Rat testis
Cellular Localization:
Chromosome, Nucleus.
Calculated MW:
67kDa
Observed MW:
90kDa
Predicted to enable chromatin binding activity. Acts upstream of or within several processes, including gamete generation; seminiferous tubule development; and synaptonemal complex assembly. Located in kinetochore; lateral element; and male germ cell nucleus. Part of nuclear meiotic cohesin complex. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; respiratory system; and sensory organ. Orthologous to human REC8 (REC8 meiotic recombination protein).
Purification Method
Affinity purification
Gene ID
56739
RRID
AB_2761885
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat testis, using REC8 Rabbit pAb (CAB15002) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
