The ~30kb positive-stranded RNA genome of coronaviruses encodes a replication/transcription machinery that is unusually complex and composed of 16 nonstructural proteins (nsps). The four proteins nsp7 to nsp10, which are conserved among all CoVs but have no functional homologs outside of the Coronaviridae, are translated as part of the viral polyproteins pp1a and pp1ab, and the mature proteins are released by the action of the SARS-CoV protease nsp5. Hexadecamer of nsp7 and nsp8 may possess dsRNA-binding activity. SARS-CoV 2 nonstructural protein 7 (nsp7) is of interest for its potential roles in the transcription and replication of the positive-stranded viral RNA genome.
Description
Background
| Information | Description |
| Product Name: | Recombinant SARS-CoV-2 NSP7 Protein (His Tag) |
| Size: | 10µg |
| SKU: | RPES0005 |
| Activity: | |
| Synonym: | SARS-CoV 2 nsp7 |
| Protein Construction: | SARS-Cov-2 NSP7 is produced by our E.coli expression system and the target gene encoding Ser1-Gln83 is expressed with a 6His tag at the C-terminus. |
| Sequence: | Ser1-Gln83 |
| Fusion tag: | C-6His |
| Accession: | YP_009725303.1 |
| Species: | virus |
| Expressed Host: | E.coli |
| Shipping: | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt; store it immediately at<-20°C. |
| Purity: | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin: | < 1.0 EU per µg as determined by the LAL method. |
| Stability and Storage: | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Mol Mass: | 12.3 kDa |
| AP Mol Mass: | 12 kDa |
| Formulation: | Supplied as a 0.2 uM filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 8.5. |
| Reconstitution: | Please refer to the printed manual for detailed information. |
