Recombinant Cynomolgus NKG2A&KLRD1/CD159a&CD94 Protein
The Recombinant Cynomolgus NKG2A&KLRD1/CD159a&CD94 Protein is a high-quality recombinant protein produced for advanced research applications in molecular biology and biotechnology. This protein serves as a critical reagent in various experimental contexts, including functional studies, binding assays, and therapeutic development programs, providing researchers with a standardized and reliable tool for investigating protein function and interactions.
This product (SKU: RPCB1739) is produced using HEK293 cells and features a C-His&Flag tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 17.01 kDa(NkG2A), 18.27 kDa(CD94) with an observed molecular weight of 25-35 kDa under denaturing conditions, achieving ≥ 95 % as determined by Tris-Bis PAGE., ensuring exceptional quality and consistency for research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant Cynomolgus NKG2A&KLRD1/CD159a&CD94 Protein
SKU:
RPCB1739
Size:
100 μg
Reactivity:
Cynomolgus
Synonyms:
CD159a, NKG2A, NKG2-A, CD94, NKG2A&CD94
Tag:
C-His&Flag
Expression Host:
HEK293 cells
Calculated MW:
17.01 kDa(NkG2A), 18.27 kDa(CD94)
Observed MW:
25-35 kDa
Protein Description:
High quality, high purity and low endotoxin recombinant Recombinant Cynomolgus NKG2A&KLRD1/CD159a&CD94 Protein (RPCB1739), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by Tris-Bis PAGE.
Reconstitution:
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
The ligand-receptor assignment between HLA-G and NKG2A/CD94 is dependent of the amino acid composition in the HLA-G heavy chain. Understanding the biophysical basis of receptor-mediated events that lead to NK cell inhibition would help to remove non-tumor reactive cells and support personalized mild autologous NK cell therapies.
Recombinant Cynomolgus NKG2A&KLRD1/CD159a&CD94 Protein was determined by Tris-Bis PAGE under reducing conditions.
Immobilized Cynomolgus NKG2A&CD94, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 8.7g/ml determined by ELISA.
