Recombinant Human 5'-Nucleotidase/NT5E/CD73 Protein (RPCB0554)
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- SKU:
- RPCB0554
- Size:
- 100µg
- Tag:
- C-hFc
- Reactivity:
- Human
- Expression Host:
- HEK293 cells
Description
| Product Name: | Recombinant Human 5'-Nucleotidase/NT5E/CD73 Protein |
| SKU: | RPCB0554 |
| Size: | 100µg |
| Tag: | C-hFc |
| Reactivity: | Human |
| Expression Host: | HEK293 cells |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human 5'-Nucleotidase/NT5E/CD73 Protein , tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 1 EU/μg of the protein by LAL method |
| Purity: | > 95% by SDS-PAGE；> 95% by HPLC |
| Formulation: | Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 120 mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
| Gene ID: | 4907 |
CD73, also known as ecto-5'-nucleotidase, is an enzyme that in humans is encoded by the NT5E gene.CD73 commonly serves to convert AMP to adenosine.The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. A? deficiency of CD73 occurs in a variety of immunodeficiency diseases.
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Swiss-Prot: | P21589-1 |
 | Human CD73 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
 | The purity of Human CD73 is greater than 95% as determined by SEC- HPLC. |
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