The Recombinant Human Amphiregulin/AREG Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Amphiregulin/AREG function and its implications in health and disease.
This product (SKU: RPCB0735) is produced using E. coli and features a No tag tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 11.4 kDa with an observed molecular weight of 16 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Human Amphiregulin/AREG Protein (RPCB0735), tested reactivity in E. coli and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.Contact us for customized product form or formulation.
Bio-Activity:
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED 50 for this effect is 5-15 ng/mL.
Reconstitution:
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Recombinant Human Amphiregulin/AREG Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
