The Recombinant Human Argonaute-2/AGO2 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Argonaute-2/AGO2 function and its implications in health and disease.
This product (SKU: RPCB0825) is produced using Baculovirus-Infected Sf9 Cells and features a N-6×His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 99 kDa, achieving ≥ 85 % as determined by SDS-PAGE., ensuring exceptional quality and consistency for research applications.
High quality, high purity and low endotoxin recombinant Recombinant Human Argonaute-2/AGO2 Protein (RPCB0825), tested reactivity in Baculovirus-Infected Sf9 Cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 85 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution 20mM Tris, 500mM NaCl, pH7.4,10% glycerol,2mM DTT.Contact us for customized product form or formulation.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
This protein is a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene.
Recombinant Human Argonaute-2/AGO2 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
