Description
Recombinant Human Asparaginyl endopeptidase/Legumain/LGMN Protein
The Recombinant Human Asparaginyl endopeptidase/Legumain/LGMN Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Asparaginyl endopeptidase/Legumain/LGMN function and its implications in health and disease.
This product (SKU: RPCB2007) is produced using Mammalian expression system and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 48.72 kDa with an observed molecular weight of 55-60 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE., ensuring exceptional quality and consistency for research applications.
Key Features
High Purity by Affinity Chromatography | |
Mammalian & Bacterial Expression Systems | |
High lot-to-lot consistency via strict QC |
Product Name: | Recombinant Human Asparaginyl endopeptidase/Legumain/LGMN Protein |
SKU: | RPCB2007 |
Size: | 50 μg |
Reactivity: | Human |
Synonyms: | Legumain, Asparaginyl Endopeptidase, Protease Cysteine 1, LGMN, PRSC1 |
Tag: | C-His |
Expression Host: | Mammalian expression system |
Calculated MW: | 48.72 kDa |
Observed MW: | 55-60 kDa |
Gene ID: | 5641 |
Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human Asparaginyl endopeptidase/Legumain/LGMN Protein (RP03221LQ), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
Endotoxin: | < 1 EU/μg of the protein by LAL method. |
Purity: | ≥ 95 % as determined by SDS-PAGE. |
Formulation: | Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 10% Glycerol, pH 8.0. |
Storage: | Store at -70℃. This product is stable at ≤ -70℃ for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles. |
Legumain is a lysosomal cysteine protease which is a member of the peptidase C13 family. Though it is found in many tissues, it is highly expressed in the kidney, heart, and placenta. Legumain has a strict specificity for hydrolysis of asparaginyl bonds and can also cleave aspartyl bonds slowly, especially under acidic conditions. Over-expression Legumain in tumors is significant for invasion and metastasis. In addition, Legumain may be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system and negative regulation of neuron apoptosis.