The Recombinant Human B7-H1/PD-L1/CD274 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating B7-H1/PD-L1/CD274 function and its implications in health and disease.
This product (SKU: RPCB1348) is produced using HEK293 cells and features a C-mFc tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 50.9 kDa with an observed molecular weight of 68-75 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE;≥95 % as determined by HPLC.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Human B7-H1/PD-L1/CD274 Protein (RPCB1348), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE;≥95 % as determined by HPLC.
Formulation:
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Bio-Activity:
Immobilized Human PD-L1 at 0.2 μg/mL (100 μL/well), dose response curve for Anti-PD-L1 Ab with the EC 50 of 1.46 ng/mL determined by ELISA.
Reconstitution:
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1 (PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.
Recombinant Human B7-H1/PD-L1/CD274 Protein was determined by Tris-Bis PAGE under reducing conditions.
The purity of Human PD-L1 is greater than 95% as determined by SEC-HPLC.
Immobilized Human PD-L1, mFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human PD-1, hFc Tag with the EC50 of 0.13μg/ml determined by ELISA.
