Description
Recombinant Human beta Defensin 3/DEFB103A Protein
The Recombinant Human beta Defensin 3/DEFB103A Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating beta Defensin 3/DEFB103A function and its implications in health and disease.
This product (SKU: RPCB1906) is produced using E. coli and features a N-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 7.3 kDa with an observed molecular weight of 12 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE., ensuring exceptional quality and consistency for research applications.
Key Features
| High Purity by Affinity Chromatography | |
| Mammalian & Bacterial Expression Systems | |
| High lot-to-lot consistency via strict QC |
| Product Name: | Recombinant Human beta Defensin 3/DEFB103A Protein |
| SKU: | RPCB1906 |
| Size: | 100 μg |
| Reactivity: | Human |
| Synonyms: | Beta-defensin 3, BD-3, DEFB-3, HBD3, hBD-3, DEFB103A |
| Tag: | N-His |
| Expression Host: | E. coli |
| Calculated MW: | 7.3 kDa |
| Observed MW: | 12 kDa |
| Gene ID: | 55894 |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human beta Defensin 3/DEFB103A Protein (RPCB1906), tested reactivity in E. coli and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | Please contact us for more information. |
| Purity: | ≥ 95 % as determined by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of 50mM Tris, 0.3% Ttiton X-100, 0.3% SKL, pH 8.5. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
Beta-defensin 3 is a member of the defensin family. Defensin family is comprised by microbicidal and cytotoxic peptides made by neutrophils. Members of the beta-defensin 3 family are highly similar in protein sequence. Beta-defensin 3 shows antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Beta-defensin 3 is abundantly expressed in skin and tonsils, and to a lesser extent in trachea, uterus, kidney, thymus, adenoid, pharynx and tongue. It is also expressed in salivary gland, bone marrow, colon, stomach, polyp and larynx. However, in small intestine, it cannot be detected. Defensin has broad spectrum antimicrobial activity and may play an important role in innate epithelial defense. Beta-defensin 3 kills multiresistant S.aureus and vancomycin-resistent E.faecium. It has no significant hemolytic activity.
 | Recombinant Human beta Defensin 3/DEFB103A Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue. |
