B-Cell Linker Protein (BLNK) is a cell membrane protein which contains 1 SH2 domain. BLNK is expressed in B cells and fibroblast cell lines, playing a important role in B cell receptor signaling. BLNK as a central linker protein, downstream of the B-cell receptor (BCR), bridges the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. BLNK associates with the activation of ERK/EPHB2, MAP kinase p38 and JNK, modulates AP1, NF-kappa-B and NFAT activation. BLNK involves in BCR-mediated PLCG1 and PLCG2 activation and Ca2+ mobilization and is required for trafficking of the BCR to late endosomes. BLNK deficiency results in agammaglobulinemia type 4 and much more profound block in B-cell development.
Product Name:
Human BLNK/Ly-57 Recombinant Protein (RPES4292)
Product Code:
RPES4292
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
B-Cell Linker Protein, B-Cell Adapter Containing a SH2 Domain Protein, B-Cell Adapter Containing a Src Homology 2 Domain Protein, Cytoplasmic Adapter Protein, Src Homology 2 Domain-Containing Leukocyte Protein of 65 kDa, SLP-65, BLNK, BASH, SLP65
Accession:
Q8WV28
Sequence:
Met1-Ser456
Fusion tag:
C-His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human BLNK is produced by our E.coli expression system and the target gene encoding Met1-Ser456 is expressed with a 6His tag at the C-terminus.
Purity:
> 90% as determined by reducing SDS-PAGE.
Mol Mass:
51.5 kDa
AP Mol Mass:
40-80 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, 1mM DTT, pH 7.2.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
