Carbonic Anhydrase X (CA10) belongs to CA family of zinc metalloenzymes; which catalyze the reversible hydration of carbon dioxide in various biological processes such as respiration; renal tubular acidification and bone resorption. While CA10 is a secreted protein without Carbonic Anhydrase activity (i.e.; the reversible hydration of CO2) due to point mutations in the zinc binding site; it has esterase activity. The human and mouse CA10 are expressed in the brain; indicating that they may play a role in brain development.
Product Name:
Carbonic Anhydrase 10/CA10 Recombinant Protein (RPES4134)
Product Code:
RPES4134
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Carbonic Anhydrase-Related Protein 10, Carbonic Anhydrase-Related Protein X, CA-RP X, CARP X, Cerebral Protein 15, CA10,CA-RPX,CARPX,HUCEP-15
Accession:
Q9NS85
Sequence:
Gln22-Asn300
Fusion tag:
C-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Carbonic Anhydrase 10 is produced by our Mammalian expression system and the target gene encoding Gln22-Asn300 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
32.8 kDa
AP Mol Mass:
34 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM TrisHCl; 150mM NaCl; pH 8.0.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
