Description
Recombinant Human Carbonic anhydrase 12 Protein
The Recombinant Human Carbonic anhydrase 12 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Carbonic anhydrase 12 function and its implications in health and disease.
This product (SKU: RPCB0273) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 31.03 kDa with an observed molecular weight of 35-40 kDa under denaturing conditions, achieving ≥ 90 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
| High Purity by Affinity Chromatography | |
| Mammalian & Bacterial Expression Systems | |
| High lot-to-lot consistency via strict QC |
| Product Name: | Recombinant Human Carbonic anhydrase 12 Protein |
| SKU: | RPCB0273 |
| Size: | 20 μg , 50 μg , 100 μg |
| Reactivity: | Human |
| Synonyms: | CA12, CA-XII, CAXII, HsT18816, T18816 |
| Tag: | C-His |
| Expression Host: | HEK293 cells |
| Calculated MW: | 31.03 kDa |
| Observed MW: | 35-40 kDa |
| Gene ID: | 771 |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human Carbonic anhydrase 12 Protein (RPCB0273), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 0.1 EU/μg of the protein by LAL method. |
| Purity: | ≥ 90 % as determined by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| Bio-Activity: | Measured by its esterase activity. The specific activity is >48 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 1 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA12, also known as Car12 and carbonic anhydrase XII, is a type I membrane enzyme that is highly expressed in normal tissues, such as colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis. It has been found to be overexpressed in 10% of clear cell renal carcinomas.
 | Recombinant Human Carbonic anhydrase 12 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue. |
