The active form of CPE cleaves C-terminal amino acid residues of the peptide, and is thus involved in the biosynthesis of peptide hormones and neurotransmitters including insulin, enkephalin, etc. It is thought that membrane-associated CPE acts as a sorting receptor for targeting regulated secretory proteins which are mostly prohormones and neuropeptides in the trans-Golgi network of the pituitary and in secretory granules into the secretory pathway. Defects in this protein are implicated in type II diabetes due to impaired glucose clearance and insulin resistance.
Product Name:
Human Carboxypeptidase E/CPE Recombinant Protein (RPES4486)
Product Code:
RPES4486
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Carboxypeptidase E(CPE for short), Carboxypeptidase H, Enkephalin convertase, Prohormone-processing carboxypeptidase
Accession:
P16870
Sequence:
Arg42-Ser453
Fusion tag:
C-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Carboxypeptidase E is produced by our Mammalian expression system and the target gene encoding Arg42-Ser453 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
47.1 kDa
AP Mol Mass:
57 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of 20mM TrisHCl,150mM NaCl,1mM ZnCl2,10%Glycerol,pH7.5.
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
