The Recombinant Human CD3E&CD3D Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating CD3E&CD3D function and its implications in health and disease.
This product (SKU: RPCB0754) is produced using E. coli and features a C-hFc tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 37.8 kDa (CD3E) , 35.4 kDa (CD3D) with an observed molecular weight of 48-58 kDa (CD3E) , 42-48 kDa (CD3D) under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC., ensuring exceptional quality and consistency for research applications.
High quality, high purity and low endotoxin recombinant Recombinant Human CD3E&CD3D Protein (RPCB0754), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.
Formulation:
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
T-cell surface glycoprotein CD3 epsilon & CD3 delta chain, also known as CD3E & CD3D, are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Recombinant Human CD3E&CD3D Protein was determined by Tris-Bis PAGE under reducing conditions.
The purity of Human CD3E&CD3D was greater than 95% as determined by SEC-HPLC.
Immobilized Human CD3E&CD3D, hFc Tag at 1 μg/mL (100ul/Well). Dose response curve for Anti-CD3E&CD3D Ab.2, mFc Tag with the EC50 of 45.9ng/mL determined by ELISA (QC Test).
Immobilized Tebentafusp, His Tag at 1 μg/mL (100 μL/well) on the plate. Dose response curve for Human CD3E&CD3D, hFc Tag with the EC50 of 8.7ng/mL determined by ELISA.
