Recombinant Human CD40 Ligand/TNFSF5 Trimer Protein
The Recombinant Human CD40 Ligand/TNFSF5 Trimer Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating CD40 Ligand/TNFSF5 Trimer function and its implications in health and disease.
This product (SKU: RPCB0686) is produced using HEK293 cells and features a N-His&Flag tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 52.4 kDa with an observed molecular weight of 55 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC., ensuring exceptional quality and consistency for research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant Human CD40 Ligand/TNFSF5 Trimer Protein
High quality, high purity and low endotoxin recombinant Recombinant Human CD40 Ligand/TNFSF5 Trimer Protein (RPCB0686), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.
Formulation:
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
CD40 ligand or CD40L, also called CD154,? is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.
Recombinant Human CD40 Ligand/TNFSF5 Trimer Protein was determined by Tris-Bis PAGE under reducing conditions.
The purity of Human CD40 Ligand Trimer is greater than 95% as determined by SEC-HPLC.
