CUB domain-containing protein 1(CDCP1) is a transmembrane glycoprotein with a large extracellular domain (ECD) containing two CUB domains; and a smaller intracellular domain (ICD) containing five tyrosines. CDCP1 is widely expressed in human epithelial tissues; but its phosphorylation is only seen in mitotically detached or shedding cells; consistent with its role in the negative regulation of cell adhesion. The tyrosine phosphorylation of CDCP1 in cultured cells occurs when cells are induced to detach by trypsinor EDTA; or seen spontaneously during mitotic detachment. The overexpression of CDCP1 leads to the loss of cell adhesion and a detached phenotype.
Product Name:
Human CDCP1/CD318 Recombinant Protein (RPES1511)
Product Code:
RPES1511
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
CUB domain-containing protein 1,Membrane glycoprotein gp140,Subtractive immunization M plus HEp3-associated 135 kDa protein,SIMA135,Transmembrane and associated with src kinases,CD318,TRASK
Accession:
Q9H5V8-3
Sequence:
Phe30-Ser341
Fusion tag:
C-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human CUB domain-containing protein 1 is produced by our Mammalian expression system and the target gene encoding Phe30-Ser341 is expressed with a 6His tag at the C-terminus.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
36.1 kDa
AP Mol Mass:
55 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB;150mM NaCl;pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
