Transcriptional Repressor CTCF (CTCF) belongs to the CTCF Zinc-Finger Protein family. CTCF contains twelve C2H2-type zinc fingers and interacts with CHD8. CTCF is highly expressed in a variety of tissues and is notably deficient in spermatocytes. CTCF is involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. CTCF is required for the development of oocytes and preimplantation embryos. It activates or silences transcription in these cells. CTCF is also important for epigenetic regulation and chromatin remodeling. Human CTCF Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human CCCTC-Binding Factor is produced by our E.coli expression system and the target gene encoding Met1-Ile154 is expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
16.9 kDa
AP Mol Mass:
33 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB,150mM NaCl,pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
