Cytotoxic T-lymphocyte protein 4; is a single-pass type I membrane protein. It is widely expressed with highest levels in lymphoid tissues. CD28 and CTLA-4; together with their ligands; B7-1 and B7-2; constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. CTLA4 transmits an inhibitory signal to T cells; whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T Cells and may play an important role in their functions. Tcell activation through the Tcell receptor and CD28 leads to increased expression of CTLA4.
Product Name:
Human CTLA4 Recombinant Protein (RPES0149)
Product Code:
RPES0149
Size:
20µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Cytotoxic T-lymphocyte protein 4,Cytotoxic T-lymphocyte-associated antigen 4,CTLA4,CD152,Cytotoxic T-Lymphocyte-Associated Protein 4
Accession:
P16410
Sequence:
Lys36-Asp161
Fusion tag:
C-Fc-Avi
Activity:
Immobilized Human CTLA-4-Fc-Avi at 2µg/ml(100 ?l/well) can bind Human B7-2-6His(Cat: PKSH033722). The ED50 of Human CTLA-4-Fc-Avi is 1.98 ug/mL
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Biotinylated Recombinant Human CTLA4 is produced by our Mammalianexpression system and the target gene encoding Lys36-Asp161 is expressed with a Fc; Avi tag at the C-terminus.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
41.5 kDa
AP Mol Mass:
57-60 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS; pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
