Stromal Cell-Derived Factor-1 (SDF-1) is a chemokine member of the intercrine family. SDF1 is expressed as five isoforms that differ only in the C terminal tail. The two major forms of SDF1 are identical except for the four amino acids present at the C-terminus of SDF1 beta but not in SDF1 alpha. CXCR4 and CXCR7 receptors are present on the cell surface for SDF1 isoforms to interact with; additionally, they may bind syndecan4. SDF-1 is known to affect lymphopoiesis, patterning and cell number of neural progenitors, and angiogenesis. It also helps myeloid progenitor cells survive. Human CXCL12/SDF Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human C-X-C Motif Chemokine 12 is produced by our E.coli expression system and the target gene encoding Ser19-Met93 is expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
8.8 kDa
AP Mol Mass:
12 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB; 150mM NaCl; pH 7.2.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
