Description
Recombinant Human CXCL4/PF-4 Protein
The Recombinant Human CXCL4/PF-4 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating CXCL4/PF-4 function and its implications in health and disease.
This product (SKU: RPCB1560) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 8.61 kDa with an observed molecular weight of 10-15 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE., ensuring exceptional quality and consistency for research applications.
Key Features
High Purity by Affinity Chromatography | |
Mammalian & Bacterial Expression Systems | |
High lot-to-lot consistency via strict QC |
Product Name: | Recombinant Human CXCL4/PF-4 Protein |
SKU: | RPCB1560 |
Size: | 10 μg , 20 μg , 50 μg |
Reactivity: | Human |
Synonyms: | PF-4, CXCL4, SCYB4 |
Tag: | C-His |
Expression Host: | HEK293 cells |
Calculated MW: | 8.61 kDa |
Observed MW: | 10-15 kDa |
Gene ID: | 5196 |
Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human CXCL4/PF-4 Protein (RPCB1560), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
Endotoxin: | < 0.1 EU/μg of the protein by LAL method. |
Purity: | ≥ 95 % as determined by SDS-PAGE. |
Formulation: | Lyophilized from a 0.22 μm filtered solution of 10 mM Sodium Citrate,150 mM NaCl,pH5.0 . |
Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
CXCL4, or Platelet factor 4 (PF4), is a small cytokine belonging to the CXC chemokine family. This chemokine is released from the alpha granules of activated platelets in the form of a homotetramer which has high affinity for heparin and is involved in platelet aggregation. CXCL4 is chemotactic for neutrophils and monocytes and also functions as an inhibitor of hematopoiesis, angiogenesis and T-cell function. CXCL4/PF4 is up-regulated in human liver fibrosis and that it plays a nonredundant, functional role in experimental liver fibrosis by mediating stellate cell proliferation, migration, and intrahepatic immune cell recruitment.