Recombinant Human/Cynomolgus/Rhesus macaque CD28 Protein
The Recombinant Human/Cynomolgus/Rhesus macaque CD28 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Recombinant Human/Cynomolgus/Rhesus macaque CD28 function and its implications in health and disease.
This product (SKU: RPCB0687) is produced using HEK293 cells and features a N-His&Avi tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 18.2 kDa with an observed molecular weight of 36-52 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE., ensuring exceptional quality and consistency for research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant Human/Cynomolgus/Rhesus macaque CD28 Protein
High quality, high purity and low endotoxin recombinant Recombinant Human/Cynomolgus/Rhesus macaque CD28 Protein (RPCB0687), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
Recombinant Human/Cynomolgus/Rhesus macaque CD28 Protein was determined by Tris-Bis PAGE under reducing conditions.
Immobilized Human/Cynomolgus/Rhesus macaque CD28, His Tag at 0.5 μg/mL (100 μL/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 of 18.4ng/mL determined by ELISA (QC Test).
Anti-CD28 antibody captured on Protein A chip, can bind Human/Cynomolgus/Rhesus macaque CD28, His Tag with an affinity constant of 5.15nM as determined in a SPR assay (Biacore T200).
