Description
| Product Name: | Recombinant Human/Cynomolgus/Rhesus macaque CD28 Protein |
| SKU: | RPCB0705 |
| Size: | 100µg |
| Tag: | N-mFc |
| Reactivity: | Human,Cynomolgus,Rhesus-macaque |
| Expression Host: | HEK293 cells |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human/Cynomolgus/Rhesus macaque CD28 Protein , tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 1 EU/μg of the protein by LAL method |
| Purity: | > 95% by SDS-PAGE |
| Formulation: | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Gene ID: | 940 |
CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Swiss-Prot: | P10747-1 |
 | Human/Cynomolgus/Rhesus macaque CD28 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
 | Immobilized Human/Cynomolgus/Rhesus macaque CD28, mFc (IgG2a) Tag at 0.5 μg/mL (100 μL/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 14.5ng/mL determined by ELISA. |
