Cytochrome b5 protein is a membrane-bound cytochrome that converts ferric haemoglobin (methemoglobin) to ferrous haemoglobin, this reaction is required for stearyl-CoA desaturase activity. Type IV Hereditary Methemoglobinemia is a disease associated with Cytochrome b5. Ambiguous genitalia and methemoglobinemia are two other disorders also linked with the CYB5A mutation. Water-soluble vitamins and cofactors are pathways dependent on the CYB5A. Human Cytochrome b5/CYB5A Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human Cytochrome b5/CYB5A Recombinant Protein (RPES1919)
Product Code:
RPES1919
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Cytochrome b5, Microsomal Cytochrome b5 Type A, MCB5, CYB5A, CYB5
Accession:
P00167
Sequence:
Met 1-Asp134
Fusion tag:
N-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Cytochrome b5 type A is produced by our E.coli expression system and the target gene encoding Met1-Asp134 is expressed with a 6His tag at the N-terminus.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
17.5 kDa
AP Mol Mass:
17 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, 0.1mM EDTA, pH 7.25.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
