The Recombinant Human DLL3 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating DLL3 function and its implications in health and disease.
This product (SKU: RPCB2086) is produced using HEK293 cells and features a N-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 50.4 kDa with an observed molecular weight of 55-60 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Human DLL3 Protein (RPCB2086), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, 200 mM Arginine, pH 7.4. Contact us for customized product form or formulation.
Bio-Activity:
Immobilized human DLL3 at 0.1 μg/mL (100 μL/well), dose response curve for Anti-Human DLL3 Ab with the EC 50 of 10.53 ng/mL determined by ELISA.
Reconstitution:
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Delta-like 3 (Drosophila), also known as DLL3, is a protein which in humans is encoded by the DLL3 gene.Two transcript variants encoding distinct isoforms have been identified for this gene.Mutations in this gene cause the autosomal recessive genetic disorder Jarcho-Levin syndrome.Expression of the gene occurs in Neuroendocrine tumors, which has been targeted as a potential pathway for treatment.
Immobilized human DLL3, His Tag at 0.1μg/mL (100 μL/well), dose response curve for Anti-Human DLL3 Ab with the EC50 of 10.53ng/mL determined by ELISA.
Recombinant Human DLL3 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
