The Recombinant Human E-Selectin/SELE/CD62E Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating E-Selectin/SELE/CD62E function and its implications in health and disease.
This product (SKU: RPCB0526) is produced using E. coli and features a C-hFc tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 85.4 kDa with an observed molecular weight of 90-130 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC., ensuring exceptional quality and consistency for research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant Human E-Selectin/SELE/CD62E Protein
SKU:
RPCB0526
Size:
100 μg
Reactivity:
Human
Synonyms:
CD62E, ELAM, ELAM1, ESEL, E-Selectin, LECAM2, SELE, selectin E, RP1-117P20.2
High quality, high purity and low endotoxin recombinant Recombinant Human E-Selectin/SELE/CD62E Protein (RPCB0526), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.
Formulation:
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha.E-selectin mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
Recombinant Human E-Selectin/SELE/CD62E Protein was determined by Tris-Bis PAGE under reducing conditions.
The purity of Human E-selectin is greater than 95% as determined by SEC-HPLC.
