The ephrin family of proteins is encoded by Ephrin A1/EFNA1. Eph receptors and related receptors, which are the most prevalent subclass of receptor protein-tyrosine kinases, have been linked to developmental processes such as nervous system development and erythropoiesis. The sequence relationships between Ephrins are classified into two categories: the ephrin-A (EFNA) class, which is glycosylphosphatidylinositol-anchored proteins, and the ephrin-B (EFNB) class, which are transmembrane proteins. Ana seqn Two different transcript variants were discovered that encode two distinct isoforms. EFNA1 is linked to cortical senile cataract and low grade glioma. Human Ephrin-A1/EFNA1 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human Ephrin-A1/EFNA1 Recombinant Protein (RPES2966)
Product Code:
RPES2966
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Ephrin-A1, EPH-Related Receptor Tyrosine Kinase Ligand 1, LERK-1, Immediate Early Response Protein B61, Tumor Necrosis Factor Alpha-Induced Protein 4, TNF Alpha-Induced Protein 4, EFNA1, EPLG1, LERK1, TNFAIP4
Accession:
P20827
Sequence:
Asp19-Ser182
Fusion tag:
C-6His
Activity:
Immobilized Human Ephrin-A1-His at 10µg/ml(100 ?l/well) can bind Human EphA2-Fc(Cat: PKSH032384). The ED50 of Human Ephrin-A1-His is 12.43ug/ml .
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Ephrin-A1 is produced by our Mammalian expression system and the target gene encoding Asp19-Ser182 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
20.4 kDa
AP Mol Mass:
24-28 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB;150mM NaCl;pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
